Expression of Bcl-2 Family in Colorectal Cancer and Inducing ...

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Cancer Research

[Abstract]

To investigate the possible roles of Bcl-2 family during the colorectal cancer development as well as to find the new targets for further therapy, we detected the expression of Bcl-2, Bcl-XL and Bax in tumor tissues and adjacent tissues for patients with colorectal cancer at both mRNA and protein levels. Our data demonstrated that Bcl-XL was dramatically higher in tumor tissues than in adjacent tissues at both mRNA and protein levels, whereas other two genes had similar expression between tumor and adjacent tissues. Next, results also showed that Bcl-2 protein was negative correlation with lymphatic metastasis and Dukes stages, it was a risk factor for overall survival as well as tumor-free survival, lack of Bcl-2 expression showed a worse prognosis. However,there is no relationship between the expression of Bcl-XL and Bax protein and clinicopathological parameters including prognosis. Furthermore, we treated DLDl cells with an artificial synthesized double-strand small interfering RNA targeting Bcl-XL (Bcl-XL siRNA). Results indicated that the level of Bcl-XL protein in DLDl cells was down-regulated by Bcl-XL siRNA effectively, and the apoptotic ratio induced by Bcl-XL siRNA was dramatically higher than that caused by control treatments , whereas the survival rate was decreased significantly.To sum up, the following conclusions could be drawn out from this study:1) Bcl-XL expression at both mRNA and protein levels were much higher than Bcl-2, suggesting it might be more important for colorectal cancer and it might be a good therapeutic target.2) Bcl-2 protein might be an indicator for prognosis evaluation.3) Bcl-XL siRNA could effectively induce the apoptosis in human colon cancer cells, suggesting it might be a new strategy for colon cancer.

Title: Expression of Bcl-2 Family in Colorectal Cancer and Inducing Apoptosis by Bcl-XL siRNA in Colon Cancer Cells

Category: Breast Cancer

Filename: Expression of Bcl-2 Family in Colorectal Cancer and Inducing Apoptosis by Bcl-XL siRNA in Colon Cancer Cells.pdf

Pages: 107

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